Patrick Adlercreutz - Forskningsoutput - Lunds universitet

Utbyte av sällsynta koder i DNA kan ge bättre - Studylib

All enzyme assays were performed by incubating 0.1 ml of diluted enzyme (0.35 to 1.0 U/ml) with 2 ml of 2% (wt/vol) soluble starch in 50 mM citric acid/disodium hydrogen phosphate buffer (pH 5.5) at 45°C for 10 min. Enzyme assays The various reactions catalyzed by B. agaradhaerens LS-3C CGTase were measured by incubating the enzyme at 50 °C with substrate solutions in 50 mM Tris–HCl buffer, pH 8.0. In all cases, the substrate was preincubated at the same temperature for about 5 min prior to addition of the enzyme. One unit of CGTase is defined as the amount of enzyme catalyzing the production of 1 μmol of β-CD/min under the reaction conditions.2.4. Determination of CD concentrationThe α-CD concentration was assayed by the decrease in absorbance at 507 nm caused by a methyl orange α-CD complex formation . CGTase enzyme production levels.

Cgtase enzyme assay

Assay of CGTase was carried out according to the method of Kaneko et al., 1987 [2]. The method is described under Analytical Methods. The amount of β-cyclodextrin produced was estimated from the standard graph of 0-500μg/mL βCD concentration against absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1μmol Catalase exhibits an unusual kinetic behavior in that it is not possible to saturate the enzyme with H2O2 substrate up to 5M catalase concentartion but there is a rapid inactivation of the enzyme at substrate concentrations above 0.1 M H2O2 . Therefore, the activity assay is typically carried out with 10-50 mM H2O2. reproducibility of the assay. PRINCIPLE: The catalase activity in a sample is determined by measuring the decrease in H 2O 2 concentration observed following an incubation of the analyte sample with an H 2O 2 standard solution.

Pontus Lundemo - Analytical Chemist - Recipharm LinkedIn

The amount of β-cyclodextrin produced was estimated from the standard graph of 0-500μg/mL βCD concentration against absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1μmol Catalase exhibits an unusual kinetic behavior in that it is not possible to saturate the enzyme with H2O2 substrate up to 5M catalase concentartion but there is a rapid inactivation of the enzyme at substrate concentrations above 0.1 M H2O2 .

Patrick Adlercreutz - Research Outputs - Lund University

The microhemagglutination assay was replaced by the more sensitive and reproducible ELISA system, which has been applied in various formats including the immobilized antigen (direct) assay , an immobilized anti-PA antibody (antigen-capture) assay , and a competition ELISA , with, where reported, varying degrees of specificity and sensitivity (17,18,28).

One unit of enzyme activity was de¢ned as the amount of enzyme that forms 1 Wmol of Q-CD from soluble starch in 1 min. Alkaline phos-phatase was assayed by the method of Yamane et al. [9]. One unit of alkaline phosphatase activity is de-¢ned as that In this case, the purity of the enzyme is important, as well as the assay procedure.On SDS-PAGE we observed three bands displaying amylolytic activity, and with IEF we observed four bands, suggesting the presence of possible degradation products of the CGTase. 2013-7-15 · CGTase assay in different temperatures, ranging from 400.C - 900C for 10 min. Then the reaction was done according to the CGTase assay.
Skyltbelysning slapvagn

29 Dec 2012 -CGTase received a lot of attention because of the Starch Plate Assay protein.

2018-08-03 Enzyme assays can be split into two groups according to their sampling method: continuous assays, where the assay gives a continuous reading of activity, and discontinuous assays, where samples are taken, the reaction stopped and then the concentration of substrates/products determined. Enzyme assays can be split into two groups: Continuous assays, where the assay gives a continuous reading of activity.
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Ngoc Trang Nhu Ngo - Lund, Sverige Professionell profil

31 Aug 2018 CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are Assay for cyclization activity of α-CGTase.